Microscope Schedule
Logbook
NYSBC Intranet
NYSBC Contacts
Cryo-EM home

NYSBC home
NMR Facility
X-Ray Facility
Protein Production Facility
Electron Crystallography Center

Cryo-EM Site map

Courses and Seminars related to CryoEM

At the NYSBC

EM Journal Club (If you are interested in presenting a paper please email cem@nysbc.org)

Past Journal Club Articles

Semester-long Course: "Cryoelectron Microscopy of Macromolecular Assemblies"

This comprehensive course will cover the theory and practice of solving molecular structures by electron microscopy. We will start with optics, sample preparation and a basic mathematical description of diffraction before moving into a detailed exploration of the three main methods of structure determination: tomography, single particle analysis, and 2D crystallography. We will end with a discussion of map interpretation and molecular fitting. Two weeks are devoted to practical sessions giving students hands-on experience with a variety of software packages for image reconstruction.

Offered every fall from September - December. 2011 Course Announcement, view flier, download flier

Local Cryo-EM Workshops and Meetings

Minisymposium on Computational Methods for Three-Dimensional Microscopy Reconstruction

The Science Center (Room 4102) The Graduate Center of the City University of New York 365 Fifth Avenue (corner of 34th St) New York, NY 10016

June 15, 2012

Program

Program Chairs: Gabor Herman (CUNY Graduate Center) Joachim Frank (Columbia University)

• 10:30-10:40 Gabor Herman (CUNY) -- Welcome
• 10:40-11:20 Yaser Hashem (Columbia)/Tryposoma brucei/ ribosome at 4.9A resolution -- a case study
• 11:20-12:00 Robert Langlois and Hstau Liao (Columbia) Preliminary results using manifold embedding
• 12:00-12:40 Bingxin Shen (Columbia) Meta-analysis of RELION-based classification results
• 12:40-14:00 Lunch break
• 14:00-14:40 Joaquin Oton (CNB, Madrid)Image formation model for soft X-ray microscopy. First 3D reconstruction attempts
• 14:40-15:20 Joanna Klukowska (CUNY) Inverting the soft X-ray transform
• 15:20-15:40 Coffee break
• 15:40-16:20 Younes Benkarroum (CUNY) Electron tomography of influenza virus by algebraic reconstruction (ART) with optimized blobs
• 16:20-17:00 Lucas Oliviera (CUNY) Using component trees to explore relationships between biological structures
• 17:00-17:40 Jose-Maria Carazo (CNB, Madrid) The new Instruct Image Processing Center, a road map of activities
• 17:40-17:50 Joachim Frank (Columbia) -- Auf Wiedersehen

This meeting is open to all and there is no registration fee. If you intend to attend, then we request (but do not insist) that you send your name, affiliation and e-mail address to gabortherman@yahoo.com

National & International Cryo-EM Meetings, Workshops and Practical Courses

2nd UB Practical course on Cryo-Electron Microscopy of Vitreous Sections (CEMOVIS)

5-7 June 2012 / 12-14 June 2012

Institute of Anatomy, University of Bern, Switzerland

Organiser : Benoît Zuber

Instructors: Benoît Zuber, Daniel Studer, Dimitri Vanhecke

The objective of the course is to teach the participants the practical skills necessary to successfully apply CEMOVIS in their laboratories. Essential background theory of CEMOVIS will be given and most of the time will be spent practicing high-pressure freezing, cryo-sectioning, and low-dose TEM imaging. 1 high-pressure freezing machine, 3 state-of-the-art cryo-ultramicrotomes and 1 cryo-electron microscope will be dedicated to the course. The 3-day course is given twice, each time with a maximum of 4 participants so that they can be actively practicing at any time.

The course is intended for scientists whose research projects will benefit from the use of CEMOVIS. Experience in either cryo-electron microscopy or ultramicrotomy of resin-embedded specimens is a prerequisite.

For details and registration please visit http://www.ana.unibe.ch/event/cemovis/

EMBO practical course on Electron Tomography in Life Science

Registration is now open for the EMBO practical course on Electron Tomography in Life Science

18-23 June, 2012, Leiden, The Netherlands

See https://electronmicroscopy.lumc.nl/embo2012.html for registration and a detailed description of the course.

Application deadline is March 31st 2012

The Fifth Brazil School for Single Particle Cryo-EM (9-21 August 2012)

This single-particle data processing school is an intensive hands-on course/workshop, in which theory and practice are tightly intertwined. The spectrum of topics covered suits attendees who are entirely new to the field as well as those who wish to acquire an in-depth understanding of the methodology. Topics range from data collection and three-dimensional reconstruction techniques to the final interpretation of the cryo-EM maps by fitting X-ray structures. The last days of the meeting will be devoted to advanced topics such as dealing with heterogeneous data sets ("4D" cryo electron microscopy) and high-resolution refinements. A special emphasis will be placed this time on the processing of massive data sets collected automatically with the newest generation of microscopes (“KRIOS”).

The attendees are expected to bring along a modern notebook computer onto which all necessary software will be installed. Most processing will take place in the context of the IMAGIC software system but other software packages such as Frealign will be used. Cryo-EM data sets will be distributed for processing during the course. The participants may bring their own data sets for processing and discussion.

The meeting will take place in the Grinberg's Village Hotel, which is situated in the scenic countryside of Socorro, about 2 hours drive from São Paulo.

For more information and registration see: www.single-particles.org/school/

EMBO practical course on cryo-EM and 3D image processing

The EMBO practical course on cryo-EM and 3D image processing will take place this year from 26th August-2nd September at EMBL in Heidelberg. As in the past, the course will provide in depth theoretical and practical training, with the focus on image processing for 3D reconstruction. Registration is now open.

Further details, including a list of confirmed teachers are available here: http://www.embl.de/training/events/2012/CRY12-01/index.html

Workshop on Image Processing with 2dx

August 19-25, 2012, in Basel, Switzerland.

We invite for applications to participate in the fourth international workshop on electron crystallography of membrane proteins. This workshop will focus on data processing, with emphasis on 3D reconstructions from images of tilted 2D crystals.

The hand-on workshop on the processing of images from cryo-electron microscopy of 2D crystals of membrane proteins (electron crystallography) will provide the theoretical background and practical applications in advanced image processing algorithms. The workshop will use the 2dx software system, which is based on the MRC programs. Theoretical morning lectures will be followed by practical exercises in the afternoon. Although we will provide iMacs for every participant, you are encouraged to bring your own laptop (Linux or OSX), or at least a portable hard-drive (USB2.0), onto which we will install the relevant software systems and test data during the workshop. You are also welcome to bring your own dataset.

Speakers and instructors include: Marcel Arheit, C-CINA, Biozentrum, University of Basel, Switzerland. Daniel Castano-Diez, C-CINA, Biozentrum, University of Basel, Switzerland Anchi Cheng, Scripps, CA, USA. Hans Hebert, Karolinska, Stockholm, Sweden. Cristina Paulino, MPI for Biophysics, Frankfurt, Germany. Sebastian Scherer, C-CINA, Biozentrum, University of Basel, Switzerland Henning Stahlberg, C-CINA, Biozentrum, University of Basel, Switzerland. Xiangyan Zeng, FVSU, GA, USA

The detailed program is available here: http://www.2dx.unibas.ch/workshop/2012

A registration fee of 300 CHF (approx. $340) for participants from non-profit organizations includes lunch and snacks. Registration is open here: http://www.2dx.unibas.ch/workshop/2012/registration-for-the-workshop

Oganizers: Marcel Arheit, Karen Bergmann, and Henning Stahlberg.

A Workshop on Advanced Topics in EM Structure Determination

The National Resource for Automated Molecular Microscopy

Announces

A Workshop on Advanced Topics in EM Structure Determination

November 11-16, 2012

National Resource for Automated Molecular Microscopy The Scripps Research Institute (TSRI), La Jolla, California.

We invite applications to participate in a Workshop on EM Structure Determination of Challenging Macromolecules. The workshop is aimed at students, postdocs and faculty that already have some experience practicing cryoEM. We can accommodate a total of 100 participants.

A major goal of the workshop will be to discuss some of the challenges that we face in using cryoEM to solve the structure of macromolecules. Topics to be discussed will include improvements in specimen preparation, imaging, processing and reconstruction, and methods for validation. The basic format of the course will be theoretical lectures in the mornings followed by practical demonstrations and panel discussions in the afternoon. Evenings will be reserved for poster presentations by participants and research talks by instructors.

The instructors for the course will include: Yifan Cheng (UCSF), Wah Chiu (Baylor), David DeRosier (Brandeis), Joachim Frank (Columbia), Bob Glaeser (LBL), Niko Grigorieff (Brandeis), Richard Henderson (MRC), Tilak Jain (TSRI), Gabe Lander (Berkeley), Steve Ludtke (Baylor), Pawel Penczek (U. Texas- Houston), John Rubinstein (Hospital for Sick Children, Toronto), Sjors Scheres (MRC), Holger Stark (Max Planck- Göttingen), Elizabeth Villa (MPI), and Tom Walz (Harvard).

Application forms may be found online at the workshop web page: http://nramm.scripps.edu/?page_id=2424

A registration fee of $750 will be charged to participants from non-profit institutions. Some partial scholarship support may be available depending on the success of efforts to raise support. All meals will be provided as part of the workshop registration fee but participants will be expected to cover their own travel and lodging expenses. Cost of housing is estimated to be under $100/night for a shared room and transportation will be provided between the hotel and the workshop venue. Further details on these and other matters will be provided once the registration process begins.

The application deadline is 1 July and a final selection of participants will be made by 15 August.

Please see all other information pertaining to the course at the web site: http://nramm.scripps.edu/?page_id=2424

For other inquiries send email to: nramm@scripps.edu

Organizers Bridget Carragher, Clinton S. Potter and Ronald A. Milligan National Resource for Automated Molecular Microscopy, The Scripps Research Institute